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Immunologia e infezioni

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Retroviral Transduction of T-cell Receptors in Mouse T-cells

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Retroviral Transduction of T-cell Receptors in Mouse T-cells

DOI:

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10:14 min

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October 22, 2010

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Shi Zhong, Karolina Malecek², Arianne Perez-Garcia, Michelle Krogsgaard

¹NYU Cancer institute,New York University School of Medicine, ²Program in Structural Biology,New York University School of Medicine

Capitoli

00:00Titolo
01:37Retroviral Transfection and T-cell Isolation
03:55Prepapration of a Single-cell Suspension from Mouse Spleen
04:51Isolation and Activation of Mouse T-cells
06:27Infection of Activated T-cells
08:21Expansion of Cells and Evaluation of Transduction Efficiency
08:55Retroviral Expression of TCR on Mouse T-cells
09:32Conclusion

Summary

Traduzione automatica

We present a protocol to produce antigen-specific mouse T-cells using retroviral transduction

Tags

Retroviral Transduction T-cell Receptors Mouse T-cells Immune System Peptide Antigens Antigen Presenting Cells Functional Outcomes Immunology Related Diseases TCR Transgenic Mouse Models Bone Marrow Chimera Retroviral Transduction Method Peptide-MHC Specificity Downstream Applications Human Immunotherapy Adoptive Transfer Cancer Treatment

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