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마우스 T - 세포에서 T - 세포 수용체의 Retroviral 전달
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Retroviral Transduction of T-cell Receptors in Mouse T-cells
DOI:

10:14 min

October 22, 2010

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Capitoli

  • 00:00Titolo
  • 01:37Retroviral Transfection and T-cell Isolation
  • 03:55Prepapration of a Single-cell Suspension from Mouse Spleen
  • 04:51Isolation and Activation of Mouse T-cells
  • 06:27Infection of Activated T-cells
  • 08:21Expansion of Cells and Evaluation of Transduction Efficiency
  • 08:55Retroviral Expression of TCR on Mouse T-cells
  • 09:32Conclusion

Summary

Traduzione automatica

우리는 retroviral 전달을 사용하여 항원 - 특정 마우스 T - 세포를 생산하는 프로토콜을 제시

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