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Journal
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Immunology and Infection
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パーコール勾配を用いて脳と脊髄単核細胞の単離
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
Please note that all translations are automatically generated.
Click here for the English version.
パーコール勾配を用いて脳と脊髄単核細胞の単離
DOI:
10.3791/2348-v
•
09:58 min
•
February 02, 2011
•
Paula A. Pino
,
Astrid E. Cardona
1
Department of Biology and South Texas Center for Emerging Infectious Diseases
,
University of Texas at San Antonio – UTSA
Chapters
00:05
Title
01:27
Preparation of Reagents
02:09
Tissue Collection and Homogenization
02:56
Gradient Set Up
05:56
Antibody Staining
08:08
Representative Analysis by Flow Cytometry
09:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
現在の記事は、効率的効果的にフローサイトメトリー分析に利用することができる脳や脊髄の組織から単核細胞を分離するために迅速なプロトコルを記述します。
Tags
Isolation
Brain
Spinal Cord
Mononuclear Cells
Percoll Gradients
Immune Cells
Central Nervous System
Infection
Trauma
Autoimmunity
Neurodegeneration
Phenotype
Effector Functions
Histochemical Approaches
Infiltrating Cells
CNS Pathology
In-situ Histochemistry
Immunofluorescent Staining Techniques
Antibodies
Immune Cell Subtypes
Histological Approaches
Flow Cytometry
Composition
Cellular Suspensions
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