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Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients

Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients

DOI:
10.3791/2348-v

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09:58 min

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February 02, 2011

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,
1Department of Biology and South Texas Center for Emerging Infectious Diseases,University of Texas at San Antonio – UTSA

Chapters

  • 00:05Title
  • 01:27Preparation of Reagents
  • 02:09Tissue Collection and Homogenization
  • 02:56Gradient Set Up
  • 05:56Antibody Staining
  • 08:08Representative Analysis by Flow Cytometry
  • 09:29Conclusion

Summary

Automatic Translation

Automatic Translation

The current article describes a rapid protocol to efficiently isolate mononuclear cells from brain and spinal cord tissues that can be effectively utilized for flow cytometric analyses.

Tags

IsolationBrainSpinal CordMononuclear CellsPercoll GradientsImmune CellsCentral Nervous SystemInfectionTraumaAutoimmunityNeurodegenerationPhenotypeEffector FunctionsHistochemical ApproachesInfiltrating CellsCNS PathologyIn-situ HistochemistryImmunofluorescent Staining TechniquesAntibodiesImmune Cell SubtypesHistological ApproachesFlow CytometryCompositionCellular Suspensions

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