Rivista
/
Medicina
/
Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
JoVE Journal
Medicina
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Medicina
Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

DOI:
10.3791/2513-v

•

10:47 min

•

February 21, 2011

•

, ,
1Department of Medicine, Pulmonary and Critical Care Medicine, Brigham and Women’s Hospital,Harvard Medical School, 2Cellular and Molecular Pathology, School of Medicine,University of Pittsburgh

Capitoli

  • 00:00Titolo
  • 01:15Dissection of Mouse Tracheas
  • 03:40Isolation of Mouse Tracheobronchial Epithelial Cells (MTEC)
  • 08:25Application of Cigarette Smoke to Cultured Epithelial Cells
  • 09:44Representative Results of Healthy Mouse Respiratory Epithelial Cell Cultures
  • 10:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

Pulmonary epithelial cells can be isolated from the respiratory tract of mice and cultured at air-liquid interface as a model of differentiated respiratory epithelium. A protocol is described for isolating, culturing and exposing these cells to mainstream cigarette smoke, in order to study molecular responses to this environmental toxin.

Tags

Mouse Respiratory Epithelial CellsIsolationExperimental Cigarette SmokeAir-liquid Interface (ALI)Differentiated Respiratory EpitheliumMainstream Cigarette Smoke (CS) ExposureAirway Epithelial CellsTracheaFully Differentiated Epithelial CellsCalibrated Mainstream CS DeliveryALI Culture SystemRespiratory EpitheliaPhysiological SettingMolecular ResponsesLung Cellular ResponsesEnvironmental Air PollutionHuman HealthChronic Obstructive Pulmonary Disease (COPD)Tobacco-related Diseases

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2513?article_type=v

Experimental Human Pneumococcal Carriage
check_url/it/2513?article_type=v

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
check_url/it/2513?article_type=v

Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice
check_url/it/2513?article_type=v

Isolation and Culture Expansion of Tumor-specific Endothelial Cells
check_url/it/2513?article_type=v

Comparing the Effects of Electronic Cigarette Vapor and Cigarette Smoke in a Novel In Vivo Exposure System
check_url/it/2513?article_type=v

Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure
check_url/it/2513?article_type=v

Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface
check_url/it/2513?article_type=v

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation
check_url/it/2513?article_type=v

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
check_url/it/2513?article_type=v

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Read Article