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Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
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Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

DOI:
10.3791/2513-v

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10:47 min

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February 21, 2011

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, ,
1Department of Medicine, Pulmonary and Critical Care Medicine, Brigham and Women’s Hospital,Harvard Medical School, 2Cellular and Molecular Pathology, School of Medicine,University of Pittsburgh

Chapters

  • 00:00Title
  • 01:15Dissection of Mouse Tracheas
  • 03:40Isolation of Mouse Tracheobronchial Epithelial Cells (MTEC)
  • 08:25Application of Cigarette Smoke to Cultured Epithelial Cells
  • 09:44Representative Results of Healthy Mouse Respiratory Epithelial Cell Cultures
  • 10:28Conclusion

Summary

Automatic Translation

Automatic Translation

Pulmonary epithelial cells can be isolated from the respiratory tract of mice and cultured at air-liquid interface as a model of differentiated respiratory epithelium. A protocol is described for isolating, culturing and exposing these cells to mainstream cigarette smoke, in order to study molecular responses to this environmental toxin.

Tags

Mouse Respiratory Epithelial CellsIsolationExperimental Cigarette SmokeAir-liquid Interface (ALI)Differentiated Respiratory EpitheliumMainstream Cigarette Smoke (CS) ExposureAirway Epithelial CellsTracheaFully Differentiated Epithelial CellsCalibrated Mainstream CS DeliveryALI Culture SystemRespiratory EpitheliaPhysiological SettingMolecular ResponsesLung Cellular ResponsesEnvironmental Air PollutionHuman HealthChronic Obstructive Pulmonary Disease (COPD)Tobacco-related Diseases

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