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Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation
JoVE Journal
Medicine
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JoVE Journal Medicine
Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

DOI:
10.3791/60812-v

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06:29 min

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May 02, 2020

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, , , , , , , ,
1Alcon Vision, LLC, 2Centre for Ocular Research and Education (CORE), School of Optometry & Vision Science,University of Waterloo

Chapters

  • 00:05Introduction
  • 00:52Cell Preparation
  • 02:26No Desiccation Protocol
  • 03:41Desiccation Protocol
  • 04:54Results: Effect of Dry Eye Lipid Enhanced Products on Cell Viability and Desiccation Protection
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

The purpose of this protocol is to evaluate if different artificial tear formulations can protect human corneal epithelial cells from desiccation using an in vitro model. After exposure to artificial tear formulations and desiccation, human corneal epithelial cells are assessed for metabolic activity.

Tags

Keywords: Corneal Epithelial CellsDesiccationArtificial Tear FormulationsMetabolic ActivityCell CultureCell Viability AssayFluorescence Measurement

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