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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
JoVE Journal
Biologia
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JoVE Journal Biologia
Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

DOI:
10.3791/265-v

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16:48 min

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August 23, 2007

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, ,
1Department of Physiology and Biophysics,University of California, Irvine (UCI), 2Department of Neurobiology and Behaviour,University of California, Irvine (UCI)

Capitoli

  • 00:00Titolo
  • 00:13Introduction
  • 00:41Tail Vein Injection for Adoptive Transfer of CFSE-labelled T Lympho
  • 03:45Removal of Lymph Nodes
  • 06:42Removing the Inguinal Lymph Nodes
  • 08:48Removing the Axillary Lymph Nodes
  • 11:24Removing the Cervical Lymph Nodes
  • 13:07Preparation of Lymph Nodes for In Situ Imaging
  • 15:362-photon Imaging of Lymph Nodes in Situ

Summary

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Two-photon imaging has uncovered lymphocyte motility and cellular interactions within the lymph node under basal conditions and durring an immune response 1. Here, we demonstrate adoptive transfer of T cells, isolation of lymph nodes, and imaging motility of CD4+ T cells in the explanted lymph node.

Tags

Dissection2-Photon ImagingPeripheral Lymph NodesMiceAdoptive TransferLabeled T CellsIsolationImaging MotilityCD4+ T CellsExplanted Lymph NodeTwo-photon Imaging Of Immune CellsFluorescently Labeled CellsCell Tracker DyeFluorescent ProteinTail Vein InjectionLymphoid OrgansIsolation Of Lymph NodeExcised Lymph NodeOxygenation Of Perfused MediaTemperatureLaser Power3D Video ImagesNaive CD4+ T Cells

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