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Capitoli
- 00:00Titolo
- 00:13Introduction
- 00:41Tail Vein Injection for Adoptive Transfer of CFSE-labelled T Lympho
- 03:45Removal of Lymph Nodes
- 06:42Removing the Inguinal Lymph Nodes
- 08:48Removing the Axillary Lymph Nodes
- 11:24Removing the Cervical Lymph Nodes
- 13:07Preparation of Lymph Nodes for In Situ Imaging
- 15:362-photon Imaging of Lymph Nodes in Situ
Zwei-Photonen-Imaging hat Lymphozyten Motilität und zellulären Interaktionen im Lymphknoten unter basalen Bedingungen und während einer Immunantwort Antwort 1 aufgedeckt. Hier zeigen wir, adoptiven Transfer von T-Zellen, Isolierung von Lymphknoten und Imaging-Motilität der CD4 + T-Zellen in der explantierten Lymphknoten.
Tags
Dissection2-Photon ImagingPeripheral Lymph NodesMiceAdoptive TransferLabeled T CellsIsolationImaging MotilityCD4+ T CellsExplanted Lymph NodeTwo-photon Imaging Of Immune CellsFluorescently Labeled CellsCell Tracker DyeFluorescent ProteinTail Vein InjectionLymphoid OrgansIsolation Of Lymph NodeExcised Lymph NodeOxygenation Of Perfused MediaTemperatureLaser Power3D Video ImagesNaive CD4+ T Cells
