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Capitoli
- 00:05Titolo
- 01:27Phosphorus Extraction and pH Adjustment
- 02:13Enzyme Stock Preparation
- 02:59P Calibration Curve and Controls
- 04:23Sample + Enzyme Incubation
- 05:20Colorimetric Measurement of Released and Background Inorganic P
- 06:09Classification of P Compounds
- 07:35HTP Analysis of Phosphorus in Soil
- 08:26Conclusion
Этот протокол описывает высокой пропускной метод ферментативного гидролиза, которая использует микропланшетного для измерения и классификации фосфора в почве, как P моноэфиры, Р диэфиры и неорганических П. До 96 образцов могут быть измерены в свое время в стандартных лабораторных условиях.
Tags
High-throughput MeasurementClassificationOrganic PhosphorusEnvironmental SamplesOrganic P CompoundsTraditional P MeasurementsColorimetric ReagentsEnzymatic HydrolysisAccurate CharacterizationP FormsPhosphorus-31 Nuclear Magnetic Resonance Spectroscopy31P-NMRExpensive MethodSpecialized Technical TrainingAdapted Enzymatic Hydrolysis MethodMicroplate Reader SystemFast And Accurate MeasurementAffordable MethodUser-friendly MeansP Species In SoilsSedimentsManuresAquatic SamplesHigh-throughput MethodForms And Enzyme-lability Of Organic PStandard LaboratorySystem Nutrient ContentEutrophication Potential
