È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:46Introduction
- 01:52Preparing the IL-1 System
- 02:42Ventilating a Mouse
- 03:34Perfusing a Ventilated Mouse
- 06:28Measuring Steady and Pulsatile Pulmonary Pressures and Flow
- 07:57Pulmonary Artery and Left Atrial Pressure Measurements
- 09:11Conclusion
The following protocol outlines the process of isolating, ventilating and instrumenting mouse lungs to measure steady or pulsatile pulmonary vascular pressure-flow relationships in order to quantify the effects of blood flow, airflow, airway changes and vascular changes on right ventricular afterload.
Tags
Isolated Mouse LungVentilated Mouse LungInstrumented Mouse LungPulsatile FlowPulmonary Vascular Pressure-flow RelationshipsPulmonary Arterial Flow RateFlow Rate WaveformAirway PressureLeft Atrial PressurePulmonary Vascular ResistanceMulti-point Pressure-flow CurvesPulmonary Vascular ImpedanceRight Ventricular AfterloadVascular CompliancePulmonary CirculationImpedance MetricsZero Hertz Impedance Z0Characteristic Impedance ZCIndex Of Wave Reflection RWDistal Arterial Cross-sectional AreaProximal Arterial StiffnessUpstream-downstream Impedance MismatchSympathetic Nervous System ToneVolume Status AnesthesiaPulmonary EmboliChronic HypoxiaVasoactive Agents
