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Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow
 

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow

Article DOI: 10.3791/2690-v • 10:03 min • April 29th, 2011
April 29th, 2011 •
1, 1
1Department of Biomedical Engineering, University of Wisconsin – Madison

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The following protocol outlines the process of isolating, ventilating and instrumenting mouse lungs to measure steady or pulsatile pulmonary vascular pressure-flow relationships in order to quantify the effects of blood flow, airflow, airway changes and vascular changes on right ventricular afterload.

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Isolated Mouse Lung Ventilated Mouse Lung Instrumented Mouse Lung Pulsatile Flow Pulmonary Vascular Pressure-flow Relationships Pulmonary Arterial Flow Rate Flow Rate Waveform Airway Pressure Left Atrial Pressure Pulmonary Vascular Resistance Multi-point Pressure-flow Curves Pulmonary Vascular Impedance Right Ventricular Afterload Vascular Compliance Pulmonary Circulation Impedance Metrics Zero Hertz Impedance Z0 Characteristic Impedance ZC Index Of Wave Reflection RW Distal Arterial Cross-sectional Area Proximal Arterial Stiffness Upstream-downstream Impedance Mismatch Sympathetic Nervous System Tone Volume Status Anesthesia Pulmonary Emboli Chronic Hypoxia Vasoactive Agents
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