Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Non-surgical Intratracheal Instillation of Mice…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunology and Infection
Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry
 

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

Article DOI: 10.3791/2702-v • 08:02 min • May 2nd, 2011
May 2nd, 2011 •
1, 2, 1, 3, 1,2,4, 1,4
1Department of Immunology, University of Colorado School of Medicine, 2Division of Cell Biology, Department of Pediatrics, National Jewish Health, 3Department of Microbiology, Immunology, and Pathology, Colorado State University, 4Department of Immunology, National Jewish Health

Capitoli

Riepilogo
Automatic Translation

We illustrate non-surgical delivery of test materials into the lungs of anesthetized mice via the trachea. This method permits lung exposure to bacterial and viral pathogens, cytokines, antibodies, beads, chemicals, or dyes. We further describe harvesting and processing of lungs and lung draining lymph nodes (LDLNs) for flow cytometry.

Tags

Non-surgical Intratracheal Instillation Mice Analysis Lungs Lung Draining Lymph Nodes Flow Cytometry Phagocytic Cells Alveolar Macrophages Lung Dendritic Cells Antigens Immune Response Inhaled Antigens T Cells Intranasal Administration Aerosolized Exposure Technical Skills Limitations Upper Respiratory Tract Nasal Associated Lymphoid Tissue (NALT) Interpretation Of Results Swallowing Sneezing Breathing Rate Of The Mouse Doses Delivered I.t Route Test Materials Blind Intubation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter