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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
 

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Article DOI: 10.3791/62670-v • 09:02 min • October 14th, 2021
October 14th, 2021 •
*1,2, *1,2, *1,2, 1,2, 1, 1,2
1Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre, University of Cambridge School of Clinical Medicine, 2Centre for Trophoblast Research, University of Cambridge
* These authors contributed equally

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This is a method to isolate uterine lymphoid cells from both pregnant and non-pregnant mice. This method can be used for multiple downstream applications such as FACS phenotyping, cell sorting, functional assays, RNA-seq, and proteomics. The protocol here demonstrates how to phenotype group 1 uterine innate lymphoid cells by flow cytometry.

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Keywords: Uterine Innate Lymphoid Cells Flow Cytometry Pregnant Non-pregnant Lymphocyte Isolation Cell Viability Fetus Removal Enzymatic Digestion EDTA PBS Cell Strainer
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