È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:35The OR-PAM System
- 04:33In Vivo OR-PAM of Mouse Ear Vasculature
- 06:48Functional OR-PAM of Total Concentration and Oxygen Saturation of Hemoglobin
- 07:23Conclusion
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
Tags
Optical-resolution Photoacoustic MicroscopyBiomedical TechnologyCellular LevelOrganelle LevelIn VivoThree-dimensional (3-D)Single-/multi-photon Fluorescence MicroscopyOptical Coherence Tomography (OCT)Optical Absorption ContrastPhysiological/pathological InformationBiomedical PhotoacousticsDiffraction LimitLateral ResolutionOptical Absorption ContrastsExtraordinary SensitivityFluorescence MicroscopyOptical-scattering-based OCTComprehensive Optical PropertiesAcoustic Waves
