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一个细胞检测系统估算的GM - CSF抗体的中和能力，采用重组可溶性GM - CSF受体
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor
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一个细胞检测系统估算的GM - CSF抗体的中和能力，采用重组可溶性GM - CSF受体

DOI:
10.3791/2742-v

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07:45 min

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June 27, 2011

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, , , , , , ,
1Bioscience Medical Research Center,Niigata University Medical and Dental Hospital, 2First department of Internal Medicine, School of Medicine,Kyorin University, 3Neosilk Laboratory,Immuno Biological Laboratories Co., Ltd.

Capitoli

  • 00:05Titolo
  • 01:49Production and Purification of sGMRalpha
  • 03:23Biotinylation of Recombinant GM-CSF
  • 04:09A Cell-free GMCSF Receptor-ligand-binding Assay
  • 05:22From Bench to Bedside
  • 07:00Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们设计了一个无细胞受体结合实验，以估计约束力的粒细胞 - 巨噬细胞集落刺激因子（GM - CSF的）受体。它使我们能够评估竞争性抑制GM - CSF的抗体生物素标记的GM - CSF的可溶性GM - CSF受体α的结合，具有优良的重现性。

Tags

Cell-free Assay SystemNeutralizing CapacityGM-CSF AntibodyRecombinant Soluble GM-CSF ReceptorAutoimmune Pulmonary Alveolar Proteinosis (PAP)Disease SeverityGM-CSF BioactivityNeutralizing Capacity AssessmentGrowth InhibitionHuman Bone Marrow CellsTF-1 CellsBioassay SystemCell-free Receptor-binding AssayTransgenic Silkworm TechnologyRecombinant SGMRαSilk ThreadsCocoons

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