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JoVE Journal Neuroscience
Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging
 
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Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging

Article DOI: 10.3791/2789-v • 11:24 min • June 22nd, 2011
June 22nd, 2011 •
1, 1, 1
1Storm Eye Institute, Medical University of South Carolina

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A method is described for the preparation of single living photoreceptor cells from different vertebrate species for fluorescence imaging. The method can be used to image the fluorescence of endogenous fluorophores, such as NADH or vitamin A, or that of exogenously added fluorescent dyes sensitive to Ca2+ or other factors.

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Living Isolated Vertebrate Photoreceptor Cells Fluorescence Imaging Phototransduction Rod Photoreceptors Cone Photoreceptors Outer Segment Visual Pigment Chromophore Opsin Photon Absorption Photoisomerization Conformational Change Cascade Of Reactions Membrane Potential Electrical Signal Recovery Process Deactivation Of Intermediates Ca2+ Modulation Adaptation To Background Light Regeneration Of Visual Pigment
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