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JoVE Journal Neuroscience
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
 

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

Article DOI: 10.3791/51477-v • 10:51 min • April 16th, 2014
April 16th, 2014 •
1, 1, 1
1Department of Cell Biology and Physiology, Washington University School of Medicine

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Four-dimensional (4D) imaging is utilized to study the behavior and interactions among two types of endosomes in living vertebrate nerve terminals. Movement of these small structures is characterized in three dimensions, permitting confirmation of events such as endosome fusion and exocytosis.

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Keywords: 4D Fluorescence Imaging Endosome Dynamics Nerve Terminals Macroendosomes Acidic Endosomes 3D Time-lapse Deconvolution Quantitative Analysis
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