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Neuroscience

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JoVE Journal Neuroscience
Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
 

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals

Article DOI: 10.3791/59694-v • 10:09 min • June 7th, 2019
June 7th, 2019 •
1,2, 1
1Department of Anesthesiology, University of Virginia Health System, 2Department of Anesthesiology, Universita' degli Studi di Padova

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We describe procedures for processing newborn rat brain tissue to obtain high-resolution electron micrographs for morphometric analysis of synaptic vesicle distribution at nerve terminals. The micrographs obtained with these methods can also be used to study the morphology of a number of other cellular components and their dimensional structural relationships.

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Keywords: Newborn Rat Brain Ultrastructural Morphometric Analysis Synaptic Vesicle Distribution Nerve Terminals Transmission Electron Microscopy Tissue Preparation Osmium Tetroxide Phosphate Buffer Uranyl Acetate Synaptic Function Synaptic Development Anesthetics
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