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Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
 

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Article DOI: 10.3791/2810-v • 13:22 min • August 18th, 2012
August 18th, 2012 •
1, 1, 1, 1,2
1The Davis Heart and Lung Research Institute, The Ohio State University, 2Department of Pharmacology, College of Medicine, The Ohio State University

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Electron paramagnetic resonance (EPR) spectroscopy was employed to detect nitric oxide from bovine aortic endothelial cells and superoxide radical anion from human neutrophils using iron (II)-N-methyl-D-glucamine dithiocarbamate, Fe(MGD)2 and 5,5-dimethyl-1-pyroroline-N-oxide, DMPO, respectively.

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Detection Nitric Oxide Superoxide Radical Anion Electron Paramagnetic Resonance Spectroscopy Cells Spin Traps Reactive Nitrogen Species Reactive Oxygen Species Cell Function Immune Response Antioxidant Defense Mechanisms Oxidative Damage Protein Function DNA Cleavage Lipid Peroxidation Oxidative Stress Cell Injury Cell Death Superoxide Radical Anion Generation Peroxynitrite Hydroxyl Radical Polymorphonuclear Neutrophils Chemotactic Stimulation Phorbol-12-myristate-13-acetate Nicotinamide Adenine Dinucleotide Phosphate Oxidase Nitric Oxide Synthase
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