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Journal / Immunology and Infection / Isolation of Fidelity Variants of RNA Viruses and …
JoVE Journal
Immunology and Infection

This Contenuto is Open Access.

JoVE Journal Immunology and Infection
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
 

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Article DOI: 10.3791/2953-v • 18:11 min • June 16th, 2011
June 16th, 2011 •
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1Viral Populations and Pathogenesis lab and CNRS 3015, Institut Pasteur

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The present article describes the steps required to isolate and characterize RNA polymerase fidelity variants of RNA viruses and how to use mutation frequency data to confirm fidelity changes in tissue culture.

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RNA Viruses Fidelity Variants Virus Mutation Frequency RNA Dependent RNA Polymerases Extreme Population Diversity Virus Adaptation Virus Evolution Intrinsic Error Rates Viral Polymerase Incorporation Fidelity Mutation Frequencies Virological Techniques Sequencing Techniques Statistical Significance Polymerase Structure And Function Virus Adaptation Mechanisms
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