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无菌实验室技术:电镀方法
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Aseptic Laboratory Techniques: Plating Methods
DOI:

18:00 min

May 11, 2012

Capitoli

  • 00:05Titolo
  • 01:51A Safe and Sterile Workplace
  • 03:04Streak Plate Procedure: Isolation of Bacterial Colonies using the Quadrant Method
  • 05:26Pour Plate Procedure: Enumeration of Bacterial Cells in a Mixed Sample
  • 06:38Spread-plating: Turntable with Glass or Metal Rod
  • 07:59Spread-plating: the Copacabana Method
  • 09:11Soft Agar Overlay Procedure: Plaque Assay
  • 11:52Replica Plate Protocol: Screening Mutants and Auxotrophs
  • 13:59Clean Up
  • 14:25Practical Applications
  • 17:22Conclusion

Summary

Traduzione automatica

媒体和试剂用于培养微生物的工作时,必须实行无菌技术,以确保污染降到最低。多种电镀方法通常用于隔离,传播,或列举细菌和噬菌体,所有这些都纳入程序,维护实验材料的无菌。

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