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Morfologiske Analyse af Drosophila Larver Perifer sensorisk Neuron dendritter og axoner anvende genetiske Mosaikker
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Neuroscience
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JoVE Journal Neuroscience
Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Morfologiske Analyse af Drosophila Larver Perifer sensorisk Neuron dendritter og axoner anvende genetiske Mosaikker

DOI:
10.3791/3111-v

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09:42 min

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November 07, 2011

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,
1Disease Mechanism Research Core,RIKEN Brain Science Institute, 2Graduate School of Science and Engineering,Saitama University

Chapters

  • 00:05Title
  • 01:13Generating Mosaics by Heat Shock
  • 02:30Screening and Imaging of Dendrite Clones
  • 03:50Larval Dissection and Tissue Preparation
  • 06:14Mounting Larval Fillets
  • 08:03Confocal Images of in vivo and Fixed Preparations
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

De dendritiske arborization sensoriske neuroner i det<em> Drosophila</em> Larver perifere nervesystem er nyttige modeller til at belyse både generelle og neuron klasse-specifikke mekanismer neuron differentiering. Vi præsenterer en praktisk guide til at generere og analysere dendritiske arborization neuron genetiske mosaikker.

Tags

Morphological AnalysisDrosophila LarvalPeripheral Sensory NeuronDendritesAxonsGenetic MosaicsNervous System DevelopmentNeuron PositionNeuron IdentityDendritic DevelopmentAxonal WiringDendritic Arborization (DA)Sensory NeuronsPeripheral Nervous System (PNS)Genetic ModelsNeuron DifferentiationClass-specific MechanismsDendritic MorphologyMolecular MechanismsFruit FlyLarval CuticleHigh Resolution VisualizationComparative AnalysisMorphometric Statistical AnalysisDA Neuron ActivityLocomotion Central Pattern Gene

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