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Journal / Neuroscience / Removal of Drosophila Muscle Tissue from Larval…
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JoVE Journal Neuroscience
Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
 

Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells

Article DOI: 10.3791/54670-v • 06:41 min • November 2nd, 2016
November 2nd, 2016 •
1, 1
1Department of Molecular Biology, Princeton University

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Summary
Automatic Translation

Studies of neuronal morphogenesis using Drosophila larval dendritic arborization (da) neurons benefit from in situ visualization of neuronal and epidermal proteins by immunofluorescence. We describe a procedure that improves immunofluorescence analysis of da neurons and surrounding epidermal cells by removing muscle tissue from the larval body wall.

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Keywords: Drosophila Muscle Tissue Immunofluorescence Larval Fillets Dendritic Arborization Da Neurons Epidermal Cells Dissection Fixation Formaldehyde PBS
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