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fjernelse af<em> Drosophila</em> Muskel Væv fra larver Filet for Immunofluorescens analyse af sensoriske neuroner og epidermale celler
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
DOI:

06:41 min

November 02, 2016

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Chapters

  • 00:05Title
  • 00:41Preparation and Dissection of Larvae
  • 02:34Fixation and Mounting of Larval Fillets
  • 04:10Results: Muscle Removal Enables Enhanced Imaging of Da Neurons and the Epidermis
  • 06:02Conclusion

Summary

Automatic Translation

Undersøgelser af neuronal morfogenese hjælp Drosophila larvestadiet dendritiske arborization (da) neuroner gavn af in situ visualisering af neuronale og epidermale proteiner ved immunofluorescens. Vi beskriver en fremgangsmåde, der forbedrer immunofluorescensanalyse af DA-neuroner og omgivende epidermisceller ved fjernelse muskelvæv fra larve kropsvæggen.

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