/

/

在体内肝细胞的分离纯化的肝细胞内吞其次是肝脏灌注

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

Please note that all translations are automatically generated. Click here for the English version.

在体内肝细胞的分离纯化的肝细胞内吞其次是肝脏灌注

DOI:

•

12:35 min

•

November 10, 2011

•

Sandhya Gopalakrishnan, Edward N. Harris

¹Department of Biochemistry,University of Nebraska, Lincoln

Capitoli

00:05Titolo
02:37Purification of Hepatocytes and SECs from Rat Liver
04:18Internalization of 125I-SA-b-Hep or Other Suitable Labeled Ligand
06:55Hepatocyte and Sinusoidal Endothelial Cell (SEC) Purification
11:08Distribution of Biotin-Labeled Heparin
12:06Conclusion

Summary

Traduzione automatica

从动物获得的无细胞系存在的主要细胞，肝窦内皮细胞（秒）的研究必须执行。这种方法依赖于肝的消化和差速离心SEC随后的培养和实验净化。

Tags

In Vivo Liver Endocytosis Purification Of Liver Cells Liver Perfusion Hepatocytes Kupffer Cells Stellate Cells Sinusoidal Endothelial Cells Fenestrae Cytoplasmic Holes Space Of Disse Scavenger Receptors SR-A Stabilin-1 Stabilin-2 Endocytosis Phagocytosis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

分离的CD133 +肝干细胞的克隆扩增

大鼠门户成纤维细胞的分离<em>原位</em>肝脏灌注

做自己动手恢复冻存样品的设备不慎掉落到低温储罐

RNA纯化的染色质隔离（CHIRP）

人肝细胞通过一个两步胶原酶灌注过程隔离

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

协议的慢病毒转导与肠组织体下游分析

一个标准化的方法肝窦内皮细胞的分析及其窗孔用扫描电子显微镜

协议主要人肝细胞的分离和相应的非实质肝细胞的主要种群

糖皮质激素粪分析：在马属动物无创监测肾上腺

Read Article