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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
 

Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions

Article DOI: 10.3791/3261-v • 08:13 min • September 2nd, 2011
September 2nd, 2011 •
1, 1
1Division of Basic Medical Sciences, Mercer University School of Medicine

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This method allows characterization of extended bacterial co-culture with EpiAirways, primary human respiratory epithelial tissue grown at the air-liquid interface, a biologically relevant in vitro model. The approach can be used with any microbe that is amenable to long-term co-culture.

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EpiAirway Model Host-pathogen Interactions Nontypeable Haemophilus Influenzae Respiratory Mucosa Bacteria Human Cells Long-term Co-culture Primary Human Respiratory Epithelial Tissues Air-liquid Interface Tight Junctions Ciliated Cells Nonciliated Cells Goblet Cells Mucin Production Cytokine Profile Bacterial Infections
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