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Immunology and Infection

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JoVE Journal Immunology and Infection
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
 

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Article DOI: 10.3791/50404-v • 13:56 min • July 18th, 2013
July 18th, 2013 •
*1,2, *1, 1, 1,3, 1
1Unité de Génétique, Papillomavirus et Cancer Humain (GPCH), Institut Pasteur, 2Cellule Pasteur, Université Sorbonne Paris Cité, 3Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology, Dana Farber Cancer Institute
* These authors contributed equally

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This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called HT-GPCA.

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Protein-protein Interactions Host-pathogen Interactions Comparative Interactomics Yeast Two-hybrid Gaussia Luciferase Complementation Assay High-throughput Screening Pathogen Strain Variants Protein Interaction Networks
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