Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Basophil Activation Test for Investigation of…
JoVE Journal
Immunology and Infection

This Contenuto is Open Access.

JoVE Journal Immunology and Infection
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
 

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Article DOI: 10.3791/3263-v • 10:22 min • September 16th, 2011
September 16th, 2011 •
1, 2, 3, 4, 5, 3, 1
1Department of Molecular Biology, University of Salzburg, 2Department of Neurology, Paracelsus Medical University, 3Department of Dermatology, Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg

Capitoli

Riepilogo
Automatic Translation

Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.

Tags

Basophil Activation Test Investigation IgE-Mediated Mechanisms Drug Hypersensitivity Non-steroidal Anti-inflammatory Drugs Propyphenazone Diclofenac Type I-like Allergic Reactions Clinical Practice Patient History Skin Testing Oral Provocation Testing IgE-mediated Pathomechanism In Vitro Method Human Basophils Drug-allergic Patients IgE Receptor Crosslinking Allergic Effector Molecules CD63 Surface Expression Flow Cytometry Low Molecular Weight Drugs Conjugates Human Serum Albumin (HSA) Carboxyl Group
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter