This content is Free Access.
Capitoli
- 00:05Titolo
- 01:40Transduction and Treatment of Target Cells
- 04:04Deconvolution from a Polyclonal Cell Population
- 06:16Target Identification and Validation
- 07:25Representative Screening Results Identifying Genes Involved in the Cytotoxicity of A549 Cells when Exposed to TRAIL and Paclitaxel
- 08:51Conclusion
Ici, nous utilisons une bibliothèque LentiPlex humains mis en commun et des méthodes de séquençage d'traditionnelle identifier les gènes cibles de promouvoir la survie cellulaire. Nous démontrons comment mettre en place et de déconvoluer un écran LentiPlex et valider les résultats.
Tags
MISSION LentiPlexPooled ShRNA LibraryScreeningMammalian CellsRNA InterferenceGene Expression RegulationKnockdownLoss Of Gene Function StudiesSmall Interfering RNAsSiRNAsShort-hairpin RNAsShRNAsPlasmidsLentiviral ParticlesLentiviral ShRNAsIntroduction Into Cell TypesLong Term Gene KnockdownSelectionHigh-throughput Loss Of Function ScreensLentiPlex Pooled ShRNA LibraryGenome-wide Lentiviral PoolTRC CollectionHuman Genes
