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Immunology and Infection
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JoVE Journal Immunology and Infection
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
 

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Article DOI: 10.3791/3322-v • 15:18 min • September 25th, 2011
September 25th, 2011 •
1, 1, 2, 3, 4, 1
1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital

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We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.

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Colorectal Cancer Cell Surface Protein Profiling Antibody Microarray Fluorescence Multiplexing CRC Prognosis Protein Expression Post-translational Modification Cluster Of Differentiation Antigens Biomarkers Immunohistochemistry (IHC) Cancer Sub-classification Prognostication Marker Clinico-pathological Staging Proteomic Techniques ITRAQ Diagnostic Laboratories
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