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Neuroscience

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JoVE Journal Neuroscience
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
 

Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

Article DOI: 10.3791/3330-v • 12:17 min • January 19th, 2012
January 19th, 2012 •
1, 2, 1
1Department of Biology, California State University, Northridge, 2Centro de Biología Celular y Molecular, Universidad Nacional de Córdoba

Capitoli

Riepilogo
Automatic Translation

An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture.

Tags

Trunk Neural Crest Cell Migration Zigmond Chamber Assay Epithelial-mesenchymal Transition Target Recognition NCC Differentiation Guidance Mechanisms Negative Guidance Cues Chemoattractants In Vitro Approaches Primary Stem Cell Cultures
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