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Patch-Clamp Kapazitätsmessungen und Ca 2 + Imaging bei Single Nervenendigungen in Retinal Slices
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Capitoli
- 00:05Titolo
- 01:37Preparation of Agar-embedded Retinal Slices
- 04:21Electrophysiological Recordings and Ca2+ Imaging
- 07:23Results: Identification and Ca2+ Imaging of Bipolar Cell Synaptic Terminals in Retinal Slices
- 08:49Conclusion
Hier beschreiben wir ein Protokoll für die Herstellung von Agar-embedded Netzhaut Scheiben, die sich für Elektrophysiologie und Ca2 +-Imaging sind. Diese Methode erlaubt es, Band-Typ Synapsen der Netzhaut Mikroschaltungen mit direkten Patch-Clamp Messungen von einzelnen präsynaptischen Nervenendigungen zu studieren.
Tags
Patch-clamp Capacitance MeasurementsCa2+ ImagingSingle Nerve TerminalsRetinal SlicesVisual StimuliDynamic RangeLight IntensitiesFrequency ChangesSpecialized NeuronsVertebrate RetinaPhotoreceptorsBipolar CellsRibbon-type Active ZonesNeurotransmitter ReleaseON-type Mixed Bipolar Cell (Mb) TerminalsGoldfish RetinaRod And Cone Photoreceptor InputRibbon-type SynapsesSize Of TerminalsLateral And Reciprocal Synaptic ConnectionsAmacrine Cell DendritesPatch-clamp TechniquePresynaptic Ca2+ CurrentsMembrane Capacitance ChangesReciprocal Synaptic Feedback InhibitionGABAA ReceptorsGABAC ReceptorsExocytosisShort-term Plasticity Of Excitatory Neurotransmitter ReleaseInhibitory Neurotransmitter Release From Amacrine Cells
