/
/
Patch-clamp Kapacitans Målinger og Ca 2 + Imaging på Single nerveterminaler i Retinal Skiver
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:37Preparation of Agar-embedded Retinal Slices
- 04:21Electrophysiological Recordings and Ca2+ Imaging
- 07:23Results: Identification and Ca2+ Imaging of Bipolar Cell Synaptic Terminals in Retinal Slices
- 08:49Conclusion
Her beskriver vi en protokol for forberedelse af agar-embedded retinal skiver, der er egnet til elektrofysiologi og Ca2 + billeddannelse. Denne metode gør det muligt at studere bånd-typen synapser i retina mikrokredsløb med direkte patch-clamp optagelser af en enkelt præsynaptiske nerveender.
Tags
Patch-clamp Capacitance MeasurementsCa2+ ImagingSingle Nerve TerminalsRetinal SlicesVisual StimuliDynamic RangeLight IntensitiesFrequency ChangesSpecialized NeuronsVertebrate RetinaPhotoreceptorsBipolar CellsRibbon-type Active ZonesNeurotransmitter ReleaseON-type Mixed Bipolar Cell (Mb) TerminalsGoldfish RetinaRod And Cone Photoreceptor InputRibbon-type SynapsesSize Of TerminalsLateral And Reciprocal Synaptic ConnectionsAmacrine Cell DendritesPatch-clamp TechniquePresynaptic Ca2+ CurrentsMembrane Capacitance ChangesReciprocal Synaptic Feedback InhibitionGABAA ReceptorsGABAC ReceptorsExocytosisShort-term Plasticity Of Excitatory Neurotransmitter ReleaseInhibitory Neurotransmitter Release From Amacrine Cells
