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Bioengineering

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JoVE Journal Bioengineering
Roterende Cell Kultur Systems for Human Cell Culture
 
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Roterende Cell Kultur Systems for Human Cell Culture: Human trophoblast Cells som en modell

Article DOI: 10.3791/3367-v • 06:54 min • January 18th, 2012
January 18th, 2012 •
*1, *1,2, 3, 1, 1
1Department of Microbiology and Immunology, Tulane University Medical School, 2Physician/Scientist Program, Tulane University Medical School, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Tradisjonell, todimensjonale cellekultur teknikker ofte resultere i endrede egenskaper med hensyn til differensiering markører, cytokiner og vekstfaktorer. Tredimensjonal cellekultur i den roterende cellekultur system (RCCS) gjenoppretter uttrykk for mange av disse faktorene som vist her med en extravillous trophoblast cellelinje.

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Bioteknologi Extravillous trophoblasts cytotrophoblast invasjon matrix metalloproteinase 3-D cellekultur RCCS ECM microcarriers
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