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Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
 

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)

Article DOI: 10.3791/3513-v • 10:59 min • April 6th, 2012
April 6th, 2012 •
1, 2,3, 1
1Centre de Recherche de l’Institut du Cerveau et de la Moelle Épinière, Hôpital de la Pitié-Salpêtrière, 2Institut des Sciences Moléculaires d'Orsay, Université Paris-Sud, 3Centre de Photonique Biomédicale du Centre Laser, Université Paris-Sud

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A technique to probe the lipid raft partitioning of fluorescent proteins at the plasma membrane of living cells is described. It takes advantage of the disparity in diffusion times of proteins located inside or outside of lipid rafts. Acquisition can be performed dynamically in control conditions or after drug addition.

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Lipid Raft Partitioning Fluorescently-tagged Probes Living Cells Fluorescence Correlation Spectroscopy Cell Membranes Microdomains Cholesterol Sphingolipids Signalling Trafficking Diseases Controversy Lipid Raft Size Microscopy Resolution Limit Direct Imaging Techniques Detergent Resistant Membranes Co-patching With Antibodies Artefacts Sensitive Analysis Plasma Membrane
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