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Whole Animal Perfusion Fixation for Rodents
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Whole Animal Perfusion Fixation for Rodents

Whole Animal Perfusion Fixation for Rodents

DOI:
10.3791/3564-v

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08:53 min

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July 30, 2012

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, ,
1Biomedical Engineering,University of Michigan , 2Department of Neurological Surgery,University of Washington School of Medicine

Capitoli

  • 00:05Titolo
  • 01:12Prepare Apparatus
  • 02:58Perfusion Surgery
  • 05:08Perfusion
  • 06:28Brain Dissection and Storage
  • 08:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.

Tags

Whole Animal Perfusion FixationRodentsFixationImmersion FixationHypoxiaPerfusing FixativeCirculatory SystemVascular NetworkPhysiological PressuresPerfusion Fixation TechniquesParaformaldehyde PerfusionBrain PreservationImmunohistochemistry

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