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赖氨酸去甲基化酶活性的定量测量中的应用的MassSQUIRM
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Biologia
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JoVE Journal Biologia
Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
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赖氨酸去甲基化酶活性的定量测量中的应用的MassSQUIRM

DOI:
10.3791/3604-v

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07:02 min

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March 11, 2012

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, ,
1Department of Biochemistry and Molecular Biology,University of Arkansas for Medical Sciences

Capitoli

  • 00:05Titolo
  • 01:34LSD1 Demethylation Assay
  • 03:16Reductive Methylation
  • 04:09MALDI Mass Spectrometry
  • 04:59Data Analysis
  • 05:32MassSQUIRM
  • 06:31Conclusion

Summary

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我们提出了一个使用MALDI质谱和还原甲基化化学的方法来量化赖氨酸甲基化的变化。

Tags

MassSQUIRMQuantitative MeasurementsLysine Demethylase ActivityEpigenetic RegulatorsSmall Molecule StudiesDrug DevelopmentHistonesProteinsDemethylase AssaysQualitative AssaysLysine Methylation StatesMass SpectrometryDifferential IonizationComprehensive AnalysisMassSQUIRM MethodIsotopic Reductive Methylation

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