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Immunology and Infection

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JoVE Journal Immunology and Infection
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
 

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

Article DOI: 10.3791/3683-v • 11:31 min • August 8th, 2012
August 8th, 2012 •
1, 1, 1
1Department of Immunology, College of Medicine, Mayo Clinic

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The chromium release assay, a common assay for detecting cytotoxic T cell activity, has several limitations. Using antigen-specific CD8 T cells and the human breast cancer tumor line, SKBR3, in the present article, an impedance-based approach was examined for the capability of detecting cell killing.

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Cytotoxic Activity CD8 T Cells Cr51 Release Assay XCELLigence System T Cell Receptor (TCR) Antigen-specific CD8 T Cells ELIspot Flow Cytometry Cytotoxicity Assay Chromium Release Assay (CRA) Gamma Radiation Reproducibility Caspase Release BLT Esterase Activity CD107 Expression Impedance-based Assay Roche XCelligence System
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