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Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
DOI:

10:31 min

September 27, 2012

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Capitoli

  • 00:05Titolo
  • 01:45Preparation of Cells
  • 02:38Pulling Glass Micropipettes
  • 03:53Microaspiration and Image Acquisition
  • 07:17Analysis
  • 07:46Results: Factors That Control Membrane Deformability
  • 10:03Conclusion

Summary

Traduzione automatica

Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.

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