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Microfluidic Mixers for Studying Protein Folding
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Bioingegneria
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JoVE Journal Bioingegneria
Microfluidic Mixers for Studying Protein Folding

Microfluidic Mixers for Studying Protein Folding

DOI:
10.3791/3976-v

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12:42 min

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April 10, 2012

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, , , ,
1Department of Physics and Astronomy,Michigan State University, 2Department of Mechanical Engineering,Hong Kong University of Science and Technology, 3Center for Biophotonics,University of California, Davis

Capitoli

  • 00:05Titolo
  • 01:05Fabrication of Microfluidic Mixing Chips
  • 05:46Mounting and Loading Chips
  • 07:35Data Collection
  • 09:55Results: Protein Folding Analysis using Microfluidic Mixers
  • 12:20Conclusion

Summary

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In this work we explain the fabrication and use of a microfluidic mixer capable of mixing two solutions in ~8 μs. We also demonstrate the use of these mixers with spectroscopic detection using UV fluorescence and fluorescence resonance energy transfer (FRET).

Tags

Microfluidic MixersProtein FoldingNative ConformationFolding KineticsStopped-flow MixersMicrofluidic MixerDilution RateLaminar FlowTurbulenceNumeric SimulationReynolds NumbersAqueous SolutionsMicron Scale GeometriesHard SubstrateChannelsOptical AccessLinear Flow RatesProtein ConsumptionProtein ConcentrationTryptophan FluorescenceFRET

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