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速度更快，高分辨率，MTPA-GFP基于线粒体融合含量的收购多个单元格的动力学数据，在并行使用共聚焦显微镜
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy
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速度更快，高分辨率，MTPA-GFP基于线粒体融合含量的收购多个单元格的动力学数据，在并行使用共聚焦显微镜

DOI:
10.3791/3991-v

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10:45 min

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July 20, 2012

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, , , ,
1Department of Neuroscience, Center for Neuroscience Research,Tufts School of Medicine, 2Department of Internal Medicine, Geriatrics & Gerontology,Wake Forest Baptist Medical Center, 3Department of Medicine,Boston University Medical Center

Capitoli

  • 00:05Titolo
  • 01:33Imaging Plate Preparation
  • 02:29Imaging Parameters for the Zeiss LSM 710 Confocal Microscope
  • 03:37Optimizing the Imaging Parameters
  • 04:49Mitochondrial-fusion Assay Set-up
  • 07:36Analyzing the PA-GFP Signal Intensity
  • 08:17Results: Representative Mitochondrial-fusion Assays
  • 09:49Conclusion

Summary

Traduzione automatica

Traduzione automatica

线粒体融合是衡量跟踪矩阵针对性photoconverted整个网络随着时间的推移线粒体的GFP平衡的。到目前为止，只有一个细胞可能受到的时间在一个小时长的动力学分析。我们提出了一个方法，同时测量多个细胞，从而加快了数据采集过程。

Tags

FasterHigh ResolutionMtPA-GFP-based Mitochondrial Fusion AssayKinetic DataMultiple CellsParallelConfocal MicroscopyFusion ActivityPhoto-conversionPhotoactivatable GFP (mtPAGFP)EquilibrationLiving CellsTime Lapse ApproachScale UpAutomateLow Concentration Of TMRESignal IntensityPAGFP Expressing CellsSubcellular QuantificationCytoarchitectural Environment

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