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Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease
JoVE Journal
Biologia
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JoVE Journal Biologia
Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

DOI:
10.3791/4137-v

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06:59 min

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September 07, 2012

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, , , ,
1Department of Pediatrics and the Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine,Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology,Indiana University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:20Inflation of Pancreas with Collagenase/Protease Mixture
  • 03:58Pancreas Dissociation
  • 05:49Results: Islet Isolation
  • 06:35Conclusion

Summary

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A detailed description of mouse islet isolation is described using the technique of in situ pancreatic ductal cannulation and perfusion of a combination of purified collagenase and neutral protease.

Tags

Mouse Islet Of Langerhans IsolationPurified CollagenaseNeutral ProteaseBeta Cell Gene ExpressionIntracellular Signaling PathwaysSecretory ResponsesIslet YieldIslet QualityRodent StrainLot-to-lot VariationsDose Of EnzymePurified TDEsRodent Cell IsolationsHuman Islet IsolationsVitaCyte LLCModified Mouse Islet Isolation ProtocolPancreatic DuctHistopaque Gradient

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