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小鼠胰岛的朗格汉斯的分离纯化的胶原酶和中性蛋白酶结合使用
JoVE Journal
Biologia
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JoVE Journal Biologia
Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease
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小鼠胰岛的朗格汉斯的分离纯化的胶原酶和中性蛋白酶结合使用

DOI:
10.3791/4137-v

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06:59 min

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September 07, 2012

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, , , ,
1Department of Pediatrics and the Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine,Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology,Indiana University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:20Inflation of Pancreas with Collagenase/Protease Mixture
  • 03:58Pancreas Dissociation
  • 05:49Results: Islet Isolation
  • 06:35Conclusion

Summary

Traduzione automatica

Traduzione automatica

小鼠胰岛分离的详细描述描述原位胰腺导管插管和灌注纯化的胶原酶和中性蛋白酶的组合中使用的技术。

Tags

Mouse Islet Of Langerhans IsolationPurified CollagenaseNeutral ProteaseBeta Cell Gene ExpressionIntracellular Signaling PathwaysSecretory ResponsesIslet YieldIslet QualityRodent StrainLot-to-lot VariationsDose Of EnzymePurified TDEsRodent Cell IsolationsHuman Islet IsolationsVitaCyte LLCModified Mouse Islet Isolation ProtocolPancreatic DuctHistopaque Gradient

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