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培养成年的背根神经节神经元轴突再生的遗传研究
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Neuroscienze
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JoVE Journal Neuroscienze
Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons
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培养成年的背根神经节神经元轴突再生的遗传研究

DOI:
10.3791/4141-v

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09:42 min

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August 17, 2012

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1Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, 2Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:50Preparing Neuronal Culture Coverslips
  • 02:37Collecting Adult Mouse DRG Neurons
  • 04:12Preparing Adult Mouse DRG Neurons for Electroporation
  • 05:40Electroporation and Culturing of Neurons
  • 08:03Genetically Manipulated Adult Mouse DRG Cultures
  • 09:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

一个<em>在体外</em用培养的成年小鼠背根神经节神经元的轴突再生的遗传研究模型描述。方法包括re-suspension/re-plating的步骤，允许进行遗传操作的神经元的轴突重新生长。这种做法是轴突再生功能丧失使用RNAi为基础的蛋白质击倒的研究特别有用。

Tags

Genetic StudyAxon RegenerationCultured Adult Dorsal Root Ganglion NeuronsCentral Nervous System (CNS)Peripheral Nervous System (PNS)Mature NeuronsAxon GrowthHostile EnvironmentPeripheral Nerve InjuriesConditioning LesionSpinal Cord InjuryMolecular MechanismsCNS Axon Regeneration

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