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人类神经内分泌肿瘤细胞株为研究人类神经内分泌肿瘤治疗的三维模型

JoVE Journal
Medicina

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JoVE Journal Medicina

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

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人类神经内分泌肿瘤细胞株为研究人类神经内分泌肿瘤治疗的三维模型

DOI:

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12:04 min

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August 14, 2012

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Chung Wong, Evan Vosburgh², Arnold J. Levine³, Lei Cong, Eugenia Y. Xu²

¹Raymond and Beverly Sackler Foundation, ²The Cancer Institute of New Jersey,University of Medicine and Dentistry of New Jersey, ³School of Natural Sciences,Institute for Advanced Study, Princeton, New Jersey

Capitoli

00:05Titolo
02:05Preparation of 1% Agarose-coated 24-well Plates
03:08Preparation of Single Cell Suspensions
04:17Spheroid Culture
05:31Drug Treatment and Monitoring of 3D Spheroid Cultures
07:22HistoGel-embedding of the NET 3D Spheroids
09:06Results: Culture of NET 3D Spheroids and Their Response to Drug Treatment
11:29Conclusion

Summary

Traduzione automatica

我们提出一个简单的琼脂糖凝胶覆盖的平台，成长的3D使用神经内分泌肿瘤细胞株的多细胞球体。这种方法提供了一个非常方便的方法来研究神经内分泌肿瘤细胞的治疗药物的效果。它也可以帮助我们建立人类癌症治疗神经内分泌肿瘤球体。

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