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3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System
 

3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System

Article DOI: 10.3791/4272-v • 08:47 min • October 25th, 2012
October 25th, 2012 •
1,2, 3, 2
1Department of Cell and Developmental Biology, Center for Stem Cell Biology, Vanderbilt University, 2Division of Gastroenterology, Hepatology, and Nutrition, Cincinnati Children's Hospital, 3Department of Biology, Duke University

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A method of visualizing and quantifying the 3-dimensional structure of mouse hepatic portal vein or intrahepatic bile duct is described. This resin cast technique can also be applied to other ductal or vascular systems and allows for in situ visualization or quantification of a system's intact communicating architecture.

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3-Dimensional Resin Casting Imaging Mouse Portal Vein Intrahepatic Bile Duct System Architecture Vascular Structures Ductal Structures Physiological Function Formation Maintenance Highly Regulated Process Analysis 2-dimensional Examination Section Dye Injection Studies Complete Representation Quantifiable Representation Novel Technique Visualizing Structures Quantifying Structures Plastic Resin Casts Permanent Snapshot Connected Structure Communicating Structure Blood Vessel Duct Network Organ Function Morphological Changes Tissue Architecture Disease States
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