Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode <em>C. elegans</em>

Jun Liang; Sheng Xiong; Cathy Savage-Dunn

doi:10.3791/4373

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Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

JoVE Journal
Biologia

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JoVE Journal Biologia

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

DOI:

10.3791/4373-v

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11:22 min

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February 13, 2013

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Jun Liang, Sheng Xiong³, Cathy Savage-Dunn³

¹Department of Science,Borough of Manhattan Community College, City Universtiy of New York (CUNY), ²Department of Biology,Queens College, The City University of New York (CUNY), ³Biochemistry Program, The Graduate Center,Queens College, The City University of New York (CUNY)

Capitoli

00:05Titolo
01:20Preparing RNAi Feeding Plates Carrying Target Gene Sequence
03:14Culture Worms on the RNAi Feeding Plates
04:58Score Body Size Phenotypes of RNAi Treated Worms
08:46Body Size Regulation by the DBL-1/TGF-β Pathway
11:00Conclusion

Summary

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We demonstrate how to use the RNAi feeding technique to knock down target genes and score body size phenotype in C. elegans. This method could be used for a large scale screen to identify potential genetic components of interest, such as those involved in body size regulation by DBL-1/TGF-β signaling.

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

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