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Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
 

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Article DOI: 10.3791/50047-v • 09:59 min • May 3rd, 2013
May 3rd, 2013 •
1,2, 2, 2,3, 4,5, 5,6, 1,2,5
1Department of Biochemistry and Molecular Medicine, University of California, Davis, 2NSF Center for Biophotonics Science & Technology, University of California, Davis, 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology), University of California, Davis, 5UC Davis Comprehensive Cancer Center, University of California, Davis, 6Department of Biological Chemistry, University of California, Davis

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Riepilogo
Automatic Translation

Autophagy is a ubiquitous process that enables cells to degrade and recycle proteins and organelles. We apply advanced fluorescence microscopy to visualize and quantify the small, but essential, physical changes associated with the induction of autophagy, including the formation and distribution of autophagosomes and lysosomes, and their fusion into autolysosomes.

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Keywords: Quantitative Analysis Autophagy 3D Fluorescence Microscopy Prostate Cancer Arginine Deprivation CWR22Rv1 Cells Autophagosomes Lysosomes Widefield Deconvolution Microscopy Structured Illumination Microscopy Digital Image Analysis
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