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Capitoli
- 00:05Titolo
- 00:58Fixing Yeast Communities
- 03:40Sectioning Frozen Communities for Fluorescence Imaging
- 05:15Results: Fluorescence Images of Vertical Cross-sections of Yeast Communities
- 06:44Conclusion
We present a protocol for freezing and cryosectioning yeast communities to observe internal patterns of fluorescent cells. The method relies on methanol-fixing and OCT-embedding to preserve the spatial distribution of cells without inactivating fluorescent proteins within a community.
